Southern African Grain Laboratory > Services > Type of Analysis

By: Sagl  11-11-2011
Keywords: Wheat

IMPORTANT INFORMATION

1.      SAGL's prices can be revised without prior notice.

 
2.      Submission of samples

 2.1         Please ensure to include the time-line required for the forwarding of samples to SAGL, as well as the lead-time required by SAGL for the testing of samples, within your internal scheduling to prevent placing SAGL under any undue pressure and to enable SAGL to present you with a reliable and accurate service.

 2.2         Each sample consignment must include an analyses request (contact information, order number, sample reference(s), list of analyses, different components of product mixtures (if applicable to your samples) and Regulatory information (if applicable to your testing). 

 2.3         Sample consignments which do not include an analyses request will be placed on hold and pending the receival of an analyses request.

 2.4         All postage, railage, airfreight, courier and other charges for delivery to the SAGL must be paid by the consignor, unless agreed differently beforehand with the General Manager of the SAGL.  SAGL will only become responsible for the sample consignment after the samples have been received.


3.      Packaging of samples

 3.1          All samples must be packaged in such a way as to prevent damage during transit. 

 3.2         Samples for moisture calibration purposes must be packaged in screw cap containers as environmental conditions can influence the sample’s moisture content.  Sample containers must be filled to the top.

 3.3          Samples for vitamin testing must be protected from sunlight and heat.

4.      General

4.1          Moisture content, protein content and mixogram is a factor in certain analyses, the fee for a single moisture, protein and/or mixogram analysis will be charged additionally per sample where applicable.  Preparation of barley samples (rub, sieve and milling) will be invoiced additionally.

4.2          Vitamin and mineral testing:

                      *Please forward any available vitamin and mineral specs with the sample consignment to assist SAGL with sample
dilutions during the preparation of samples for vitamin analyses. 
*Please inform the SAGL should you submit samples for vitamin or mineral analysis that you suspect might fall outside 
the specs of the fortification regulations, as this will assist SAGL in the evaluation of the test results and prevent 
unnecessary repeat analysis for confirmation purposes. Please also note that additional invoicing will be applicable 
for additional dilutions as this will result in an additional analysis to obtain a test result. 

        4.3          The test results of calibration samples cannot be guaranteed without homogeneity testing.

 4.4          Test results relate only to the samples tested. 

 4.5         Should a specific matrix not be listed in the method’s scope and field of application, the following note will be documented on the test report:  Product does not form part of the method's scope.

 4.6          Uncertainty of measurement values for specific analytical methods are available on request.

 4.7           If available, sealed arbitration samples are stored for one month by the SAGL for possible future enquiries.

 4.8          Test report may not be reproduced, except in full.  When only certain pages or sections of the full report are reproduced, written permission must be given by the General Manager.

 4.9          The SAGL or its members shall in no way be liable for any error made when carrying out a test or erroneous statement, whether in fact or in opinion.

5.      Financial

         5.1        Please note that all prices documented are excluding VAT.

         5.2        Samples received without an order number will be placed on hold until the order number has been received.

         5.3        SAGL’s Policy for new clients require upfront payment for test reports during the first 6 months and thereafter on a 30 day account, subject to the completed 
documents received. Terms are strictly 30 days and must be paid on the receipt of statement.   

         5.4        Foreign customers must settle their account and the payment has to reflect on our banking account before report can be forwarded.

         5.5       Preliminary reports will be invoiced at an additional fee.

         5.6       Single-Sample-Price Principal:  The single-sample-price applies when a customer submits only one sample for certain analyses. 

         5.7       Sliding scale prices will be implemented where applicable on analyses/projects.

Wheat Grading

Analysis particulars

Full wheat grading without cultivar identification / (2.5kg sample)

Hectolitre mass (minimum mass 1.5kg)  (Method SANAS Accredited)         

Ergot (2.5kg sample), %

Foreign matter, %

Heat damaged kernels, %              

Immature kernels, %                                                                       

Insect damaged kernels, %            

Sprouted kernels, %         

Heavily frost damaged kernels, %

Fungi (field/storage), %                                   

Noxious seeds (10kg sample required), Total / 10kg

Other grain and unthreshed ears, %             

Screenings, % (1.8mm) (Method SANAS Accredited)                                                         

Undesirable odours                                                         

 Hectolitre mass is carried out according to ISO 7971-3,2009 (E). Remaining wheat grading is carried out according to Government Gazette grading regulations
Minimum mass: 2.5kg

Maize Grading

Analysis particulars

Full maize grading

Defective kernels (Method SANAS Accredited)

Lemon Yellow, %

 Full maize grading is carried out according to the Government Gazette grading regulations.
Minimum mass: 2kg

Other Grading

Analysis particulars

Grading of sunflower (minimum mass: 1kg)
Grading of Soya (minimum mass: 2kg)

Physical Tests

Analysis particulars

Reference

100 Kernel mass (maize), g

Industry Accepted Method 001

1000 Kernel mass (wheat), g

Industry Accepted Method 008

Breakage susceptibility,  g < 6.35mm, g < 4.75mm

Industry Accepted Method 007

Kernel size (10mm, 8mm, 6.35mm, 4.8mm, 2.4mm) (Maize), %

Industry Accepted Method 017

Pap cooking test

Industry Accepted Method 005

Granulation (Wheat flour), % (Particle size)

Industry Accepted Method 003

Granulation (Maize products), %(Particle size)

Industry Accepted Method 003

Screenings  (Wheat) (USA), % (1.6mm)

U.S. Department of Agriculture, GIPSA, Federal Grain Inspection Handbook II, Wheat, 6/1/97

Sieve test (Maize) (USA), % (4.8mm, 2.4mm)

U.S. Department of Agriculture, GIPSA, Federal Grain Inspection Handbook II, Corn, 6/1/97

Speck count , 100cm2       

Industry Accepted Method 002

Stress cracks, %

Industry Accepted Method 006

Milling

Analysis particulars

Reference

Bühler  Extraction (2‑6kg)

Industry Accepted Method 011

Cleaning of wheat on the Dockage Tester

Before milling

Vitreous kernels, %

Industry Accepted Method 009

Quadromat milling (Durum)

Industry Accepted Method 024

Quadromat milling (Wheat)

Industry Accepted Method 024

Roff 150 (Maize)

Industry Accepted Method 013

Chemical Analysis

Analysis particulars

Reference

Ash, %  (Moisture is a factor)

In-house Method 011 (Method SANAS Accredited)

Bran content, % (212 µm)

Government Notice R. 186 of 22 February 2008

Colour, Kent Jones (KJ)   

FTP Method No. 0007/3, 7/1991

CALCULATIONS

Carbohydrates, g/100g (Calculated value)

SOP MC23 (SOP SANAS Accredited)(The following analyses is a factor in the calculation:  Moisture, Fat, Protein, Ash and Fibre (Crude for animal consumption or Dietary for human consumption))

Baking worth

Analyses required for calculation:  Moisture, protein, Farinogram, Alveogram, Mixogram, 100g baking test

Energy value, kJ/100g

(Calculated value)

SOP MC23 (SOP SANAS Accredited) (The following analyses is a factor in the calculation:  Moisture, Fat, Protein, Ash and Fibre (Crude for animal consumption or Dietary for human consumption))

Total digestible nutritional value, % 

(Calculated value)

SOP MC23 (SOP SANAS Accredited) (The following analyses is a factor in the calculation:  Moisture, Fat, Protein, Ash and Fibre (Crude for animal consumption or Dietary for human consumption))

Milling Worth

Analyses required for calculation:  Hectolitre mass, 1000 kernel mass, Büler milling, KJ colour, moisture, Falling Number

Additional calculations on request (E.g.:  as is, dry basis, 12 % moisture basis)

Damaged starch, % (Moisture is a factor)     

In-house Method 23

Diastatic activity, mg/10g (Maltose)

In-house Method 25

Drying and milling of bread

Government Gazette Regulation No. 13074, R.577, 15 March 1991 / FN milling:  ICC Std No. 107/1, Latest Edition

Dry solids determination of bread

Government Notice R. 186 of 22 February 2008

Falling Number, sek.  (Moisture is a factor / minimum 300g sample)

ICC Std No. 107/1, Latest Edition (Method SANAS Accredited)

Fat Crude (Petroleum ether extraction) (Soxhlet) , %   (Moisture is a factor)

In-house Method 024 (Method SANAS Accredited)

Fibre Crude (Acid–base digestion – 3 days), % (Moisture is a factor) 

In-house Method 20

Fibre Dietary (total), % (g/100)

In-house Method 012 (Method SANAS Accredited)

GMO (Bt-Cry1Ab – maize and maize meal)

SDI Bt maize Kit User's Guide, Rev. 011601, Ver. 1.4 (ELISA)

GMO (RUR-NK603 – maize and maize meal)

SDI Soya Test Kit with RUR NK603 Maize Testing Protocol(ELISA)

GMO (RUR-CP4 – Soya beans and ground Soya beans)

SDI Soya Test Kit User's Guide, Rev. 031703, Ver. 2.1 (ELISA)

Hunterlab colour, Lab

Industry Accepted Method 004

Milling index (Maize), Infratec (NIT)

Industry Accepted Method 015

Moisture (Barley – 2 hour oven milled kernels), % (130 – 133 °C) v

Analytica EBC Method 3.2, Latest Edition (Method SANAS Accredited)

Moisture (Canola – 17 hour oven whole kernels), % (103 °C)

ISTA, Section 9, Latest Edition

Moisture (milled Maize – 1 hour oven), %(130 ° C)               

AACC 44-15.02, Latest Edition (Method SANAS Accredited)

Moisture (milled Soya beans – 17 hour oven), %(103 °C)               

ISTA Handbook, Section 9, Latest Edition

Moisture (Maize/Soya beans – 72 hour oven whole kernels / beans,  % (103 oC)

AACC 44-15.02, Latest Edition (Method SANAS Accredited)

Moisture (Malt – 3 hour oven milled kernels), % (106 °C)

Analytica EBC Method 4.1, Latest Edition

Moisture (Sorghum – 2 hours oven milled kernels), % (130 °C)

ISTA, Section 9, Latest Edition

Moisture (Sorghum – 72 hours oven whole kernels), % (103 °C)

Sorghum Grading Regulations No. R.573 (Paragraph 19) (AACC 45-15.02)

Moisture (Sunflower – 3+ hour oven whole seeds), % (103°C)

ISO 665 – 1977 E

Moisture (Wheat – 72 hour oven whole kernels), % (103 oC)

Government Gazette No 33860 dated 17 December 2010, Regulation No.R.1186 (Method SANAS Accredited)

Moisture (Wheat/Rice/Millet milled kernels – 2 hour oven), % (130 ° C)          

ICC Std No. 110/1, Latest Edition (Method SANAS Accredited)

Moisture (Wheat flour – 90 minutes oven), % (130 ° C)         

ICC Std No. 110/1, Latest Edition (Method SANAS Accredited)

Moisture (Vegetable seeds), %

ISTA Section 9, Latest Edition (Whole seeds or milling, oven duration and temperature differs between species)

pH

AACC 02-52.01, Latest Edition

Protein (crude) – other, % Dumas (Leco)  (Moisture is a factor) 
Protein (crude) – on wet samples

AACC 46-30.01, Latest Edition (Method SANAS Accredited)

Starch content, % (Polarimeter) (Moisture is a factor)

In-house Method 019

Whiteness index  87:13 sifted (300µm)

Industry Accepted Method 004

Whiteness index unsifted

Industry Accepted Method 004

Whiteness index unsifted & sifted

Industry Accepted Method 004

Mycotoxins

Analysis particulars

Reference

Determining the Multi-Mycotoxins using  UPLC-MS/MS: Includes Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Fumonisin B1, T2-Toxin, Deoxynivalenol, Ochratoxin A and Zearalenone

In-house Method 026 (UPLC-MS/MS) (Method SANAS Accredited)

Aflatoxin (total) (Sensitivity 0.1 ppb to 300 ppb) ppb/dpb = µg/kg

ROSA Quantitative Method, Latest Edition

Deoxynivalenol (DON), (Limit of detection:  0.50 ppm to 5.0 ppm) ppm/dpm = mg/kg

ROSA Quantitative Method, Latest Edition

Fumonisin (Sensitivity 0.25 ppm to 10 ppm) ppm/dpm = mg/kg

ROSA Quantitative Method, Latest Edition

Ochratoxin A, (Sensitivity 1 ppb to 100 ppb) ppb/dpb = µg/kg

ROSA Quantitative Method, Latest Edition

Zearalenone (Sensitivity 0.1 ppm to 5 ppm) ppm/dpm = mg/kg

ROSA Quantitative Method, Latest Edition

HPLC and AA

Analysis particulars

Reference

AMINO ACIDS – Sliding scale prices will be implemented where applicable on analysis/projects

Protein bound amino acids (Foodstuffs)

In-house Method 009 (HPLC)

Protein bound amino acids (Pharmaceutical)

In-house Method 009 (HPLC)

Tryptophan

In-house Method 007 (HPLC)

Cysteine and/or Methionine

In-house Method 015 (HPLC)

Melamine  – Sliding scale prices will be applicable on more than 1 sample received simultaneously

HPLC determination (positive results must be verified using mass spectrometry)

In-house method IH 022 (HPLC)

Confirmation of positive results: Samples will be subcontracted

Please note the following: 
-  The verification of positive test results will be at an additional fee and additional lead-time
- The lead-time will be determined by the subcontracted laboratory.  Should customers request that SAGL negotiate a shorter lead-time with the subcontracted laboratory, an urgency rate may be applicable. Cost will include the following: 
-  Cost as quoted by subcontracting laboratory
-  R300.00 handling fee
- Courier cost for forwarding of sample to the subcontracting laboratory

SUDAN RED – price for 1 to 10 samples.  11+ samples:  price  will be determined according to a sliding scale

Package for Sudan I, II, III and IV/Para Red

Minimum 10 samples per analyses request

In-house Method 005 (HPLC)

VITAMINS – Sliding scale prices will be implemented where applicable on analysis/projects

Ascorbic Acid (Vitamin C)

 In-house Method 016 (HPLC)

Folic Acid (Vitamin B9)  (Moisture is a factor when testing according to the fortification regulation)

In-house Method 003 (HPLC) (Method SANAS Accredited)

Nicotinamide

In-house Method 002 (HPLC) (Method SANAS Accredited)

Pyridoxine (Vitamin B6) (Moisture is a factor when testing according to the fortification regulation) 

In-house Method 002 (HPLC) (Method SANAS Accredited)

Riboflavin (Vitamin B2) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 002 (HPLC) (Method SANAS Accredited)

Thiamine (Vitamin B1) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 002 (HPLC) (Method SANAS Accredited)

Vitamin A (Retinol saphonication)  (Moisture is a factor when testing according to the fortification regulation)

In-house Method 001 (HPLC) (Method SANAS Accredited)

Package for Fortification (Vitamins A, B1, B2, B6, B9, Nicotinamide) (Moisture is a factor when testing according to the fortification regulation) 

Vitamins:  HPLC In-house Method 001 – 003

MINERALS – Sliding scale prices will be implemented where applicable on analysis/projects

Iron – Qualitative Method (Yes/No)

AACC 40-40.01, Latest Edition

Iron (Total) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 010 (AA) (Method SANAS Accredited)

Zinc (Total) (Moisture is a factor when testing according to the fortification regulation)

In-house Method 010 (AA) (Method SANAS Accredited)

VITAMIN AND MINERAL:  Regulations relating to the fortification of certain foodstuffs, 7 April 2003.  Government Gazette No. 24715 – Wheat flour 14%mb / Maize meal 12.5%mb.

*Please protect samples from sunlight and heat.

*Please forward any available vitamin and mineral specs with the sample consignment to assist SAGL with sample dilutions during the preparation of samples for vitamin analyses.

 *Please inform the SAGL should you submit samples for vitamin or mineral analysis that you   suspect might fall outside the specs of the fortification regulations, as this will assist SAGL in the evaluation of the test results and prevent unnecessary repeat analysis for confirmation purposes. Please also note that additional invoicing will be applicable for additional dilutions as this will result in an additional analysis to obtain a test result.

  Rheological and Baking

Analysis particulars

Reference

Alveogram (Moisture is a factor)      

ICC Std No. 121, Latest Edition (Method SANAS Accredited)

Alveogram Extended Method (Moisture is a factor)                       

Application Notes for the Chopin Alveograph, Note No. 8, p.21

Baking test, (100 g) (Moisture, protein content and mixogram is a factor)   

Industry Accepted Method 022

Baking test (commercial) with or without drop test

Industry Accepted Method 018

Consistogram (Moisture is a factor)  

AACC 54-50.01, Latest Edition

Extensigram 45 minutes 
(Moisture is a factor)               

ICC Std No. 114/1, Latest Edition

Extensigram additional 90 minutes 90 minute       

ICC Std No. 114/1, Latest Edition

Farinogram  (Moisture is a factor)   

AACC 54-21.01, Latest Edition – Constant Flour Weight Procedure (Method SANAS Accredited)

Gluten (flour – wet and/or dry) (Moisture is a factor)  

AACC 38 – 12.02, Latest Edition

Gluten Index (Moisture is a factor)  

AACC 38 – 12.02, Latest Edition

Mixogram (flour or whole wheat flour)  (Moisture and protein content is a factor)  

Industry Accepted Method 020 (Method SANAS Accredited)


Keywords: Wheat

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Remaining wheat grading is carried out according to Government Gazette No. 33860 dated 17 December 2010, Regulation No. R.1186. Hectolitre mass (Kern 222) is carried out according to ISO 7971-3. Foreign matter, % (Including Gravel, stones, turf and glass. SAGL WHEAT GRADING RING TEST.